Plasmid

Part:BBa_K5348028:Design

Designed by: ER DU   Group: iGEM24_Songshan-Lake   (2024-09-23)


pHT43-pL-RBS3-mcherry


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1091
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3022
    Illegal BglII site found at 6103
    Illegal BamHI site found at 2905
    Illegal BamHI site found at 9975
    Illegal XhoI site found at 6107
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 8688
    Illegal NgoMIV site found at 8760
    Illegal NgoMIV site found at 8850
    Illegal NgoMIV site found at 8868
    Illegal NgoMIV site found at 9360
    Illegal NgoMIV site found at 9653
    Illegal NgoMIV site found at 9747
    Illegal AgeI site found at 2932
    Illegal AgeI site found at 8402
    Illegal AgeI site found at 9528
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9417
    Illegal BsaI.rc site found at 8301


Design Notes

NO


Source

NO

References